FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93912478
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC13468031.434104422204683No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT5005430.5329888111354063No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2945760.3136707775935803No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1813620.19311810726578846No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1579740.16821406842230274No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1272850.13553576980473245No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1191900.12691604197687126No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1155410.12303050932166862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC21608400.069.5252712
CCTGTAC21438050.069.497042
TGAGCTG21572750.069.457669
TCCTGTA21404050.069.444881
TGTACTG21392250.069.443834
GAGCTGC21734600.069.4083210
CTGTACT21411700.069.388563
TGCCCCG21634750.069.3455314
CTGCCCC21667300.069.2769913
ACTGAGC21718450.069.207297
GTCTCGG2263800.069.19166611
TGTCAGT2866350.069.124591
GTACTGA21566950.069.099965
AGCTGCC21765000.069.0972911
GCCCCGA21749300.069.0509115
GTTGTAT967700.068.96255512
GCAGCAC6948600.068.935843
GTAAATA6491150.068.9266510
CTGAGCT21765600.068.869298
TAGGTTG1426000.068.862849
Produced by FastQC (version 0.11.5)