FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_86_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_86_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18488707
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1357880.7344375136671266No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC538340.29117233563169126No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA251070.13579640804519214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC5788150.069.6453110
GCTGCCC5789450.069.64236512
TCCTGTA5767400.069.587461
CCTGTAC5776200.069.585082
CTGTACT5770400.069.580833
TGAGCTG5789150.069.5710149
GTACTGA5777600.069.561225
TGTACTG5773050.069.560874
CGTAAAT2153150.069.556919
AAATATT2152100.069.5355512
ACTGAGC5802100.069.523717
TTGGCGA2038150.069.4945617
TGGCGAA2035600.069.492218
GTAGGTT402850.069.486468
CAGCACG2153400.069.482954
GTAAATA2157600.069.4734710
TATTGGC2092300.069.4604115
TGCCCCG5799250.069.45286614
ATATTGG2153700.069.4237614
CTGAGCT5806150.069.422778
Produced by FastQC (version 0.11.5)