FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_85_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_85_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17679257
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2165561.2249157303386675No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1290810.7301268373438997No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC540630.3057990502655174No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA477980.2703620406672068No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC292060.16519925017210849No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA288270.16305549492266558No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG250170.1415048155021447No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG211880.11984666550183642No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT192070.10864144347242648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC4455550.069.6502210
GCTGCCC4452600.069.63912
CCTGTAC4445250.069.6010062
TGAGCTG4453050.069.592669
CTGTACT4440750.069.558433
TGTACTG4441100.069.55634
GTACTGA4447250.069.540385
TGTCAGT1428300.069.522591
ACTGAGC4466400.069.517867
TGCCCCG4459350.069.429214
CTGCCCC4466600.069.3298313
CTGAGCT4473200.069.326128
GCCCCGA4463100.069.2971515
TCCTGTA4461850.069.2282941
GTCTCGG596400.069.22436511
GGCAGTT224700.069.2046211
CGTTACC1680150.069.199295
CCGTTAC1678900.069.1986854
GTTACCA1681150.069.1268846
CGTAAAT726600.069.112699
Produced by FastQC (version 0.11.5)