FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_81_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_81_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19287127
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC6515433.3781236572974294No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1433330.7431537107626243No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1077400.5586109325665767No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC967260.5015054860166577No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA702740.3643570138777019No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT696030.36087800946195875No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT678170.3516179470379388No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG583440.30250228559183545No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG329890.17104154496416185No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC327060.16957424503919116No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG259380.13448348216922096No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG203590.10555745290628303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5974350.069.48093412
GAGCTGC5977900.069.47832510
CCTGTAC5957700.069.454972
TGAGCTG5977000.069.449
CTGTACT5953600.069.4198763
TGTACTG5955200.069.417824
GTACTGA5962050.069.415565
TCCTGTA5954350.069.406431
ACTGAGC5989150.069.400827
TGCCCCG5980600.069.2971414
GTCTCGG590750.069.2641711
CTGAGCT5997350.069.238238
CTGCCCC5988450.069.2355213
GCCCCGA5985000.069.1789415
TGTCAGT728650.069.1163941
AGCTGCC6016050.069.0039911
TCTCGGT591250.068.9451312
TATTGCA1332450.068.884621
CGTTACC1110800.068.88175
TGTCTCG593700.068.8787410
Produced by FastQC (version 0.11.5)