FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_77_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_77_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21295599
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC6667473.1309145143088015No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1448130.6800137436847867No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC935390.4392409905915302No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA751070.35268789574784914No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT728420.3420518953235361No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA664350.3119658667502144No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT640200.30062549543687406No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG582670.2736105239397117No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG373120.1752099107425905No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG327240.15366555315020722No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG222990.10471177636280622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT3124550.069.5273749
TATTGGC3019150.069.5271415
CAGCACG3124000.069.480894
TTGGCGA2923050.069.4700517
GTAAATA3129650.069.45209510
TGAGGTA1293050.069.4490361
GTAGGTT691600.069.447348
TGGCGAA2920400.069.440818
TAGCAGC3457450.069.438081
ATATTGG3124700.069.4299514
GCACGTA3129650.069.428156
AAATATT3125700.069.42229512
ATTGGCG2949650.069.42007416
GCTGCCC2799000.069.3887412
GCAGCAC3461300.069.3842543
AGTAGGT692600.069.382457
GAGCTGC2802200.069.3532210
TGTCAGT1083050.069.351181
GTTGTAT499150.069.318812
CACGTAA3134700.069.306727
Produced by FastQC (version 0.11.5)