FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_75_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_75_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23384450
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2355601.0073360716202435No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT507130.21686633639020803No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC409930.17530025294586787No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC328800.14060625757715062No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA282710.12089657870935601No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT278640.1191561058737751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT1358500.069.351631
GTCTCGG872500.069.2770711
GCTGCCC4945700.069.12726612
GAGCTGC4952200.069.110
GTAGGTT397050.069.0557868
CCTGTAC4948350.069.047262
TGTACTG4943400.069.032144
TGAGCTG4953250.069.02679
GTACTGA4948100.069.011135
CTGTACT4943950.069.008683
TCCTGTA4948750.068.979421
ACTGAGC4966000.068.9580157
TCTCGGT873550.068.9093412
AGTAGGT397950.068.899617
TGTCTCG876950.068.8934610
CATTGCA877450.068.871431
TGCCCCG4955000.068.8548414
GGCAGTT396800.068.81718411
CTTGTCT877600.068.806548
CAACGGA1947350.068.774261
Produced by FastQC (version 0.11.5)