FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_70_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_70_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16410296
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC9451525.759506105191521No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1891081.152374094897496No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1564270.953224731595335No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1449630.8833661501291628No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1032430.6291355134605737No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG978160.596064811993641No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC728130.44370314831615465No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT663580.4043680869619902No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA548650.3343327871721509No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA502480.30619801129729773No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG362980.22119040387815064No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG282850.1723613029283567No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA276470.16847349980768173No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG262940.16022867594831927No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA211870.1291079697770229No Hit
TCGCTATGATGATGGATTCCAAAACCATTCGTAGTTTCCACCAGAAAGTC202410.12334329618429796No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT200290.12205142430093886No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA183420.11177129285175601No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG182960.11149098102800827No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAA172910.10536677705265036No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT172790.10529365222906399No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGC166020.10116819343173335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC3069350.069.3444512
TCCTGTA3065200.069.22881
GAGCTGC3077750.069.167710
TGCCCCG3074550.069.1304114
CCTGTAC3074000.069.1242
GTAGGTT179000.069.0995568
TGAGCTG2989500.069.0963449
CTGTACT2983750.069.057033
CTGCCCC3079200.069.04420513
TGTACTG2984550.069.029264
GTTGTAT123550.068.9791712
GTACTGA2989850.068.968945
GCCCCGA3079150.068.96348615
GTCTCGG301400.068.872611
AGTAGGT179600.068.849227
TAGGTTG179050.068.845699
ACTGAGC3008950.068.766017
TTGGCGA345550.068.7632917
GGCAGTT211950.068.7439911
CAACGGA1151950.068.7412341
Produced by FastQC (version 0.11.5)