FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_69_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_69_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18239220
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC4737162.59723825909222No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1777140.9743508768467073No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC994060.545012341536535No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC733500.4021553553276949No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA643530.3528275880218562No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT609240.3340274419629787No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA530840.29104314767846434No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG424180.23256476976537374No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC239460.13128850904808428No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG208630.11438537393594682No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG199350.10929743706145328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG531150.069.0964411
GCTGCCC4313000.069.0408212
TGTCAGT890900.069.0265961
GAGCTGC4317300.068.97529610
CCTGTAC4306750.068.9471052
TGTACTG4303950.068.922274
TGAGCTG4317150.068.91779
CTGTACT4303500.068.915973
GTACTGA4307700.068.9150855
TCCTGTA4303100.068.890871
ACTGAGC4327500.068.874197
GTACAGG181500.068.86146510
GGCAGTT321150.068.7894811
TGCCCCG4323250.068.7726914
GTAGGTT206300.068.760728
CTGCCCC4327600.068.7100413
TGTCTCG533750.068.7008410
CGTAAAT567950.068.692769
GGGCTAT160850.068.6501215
GCCCCGA4325600.068.6471415
Produced by FastQC (version 0.11.5)