FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_64_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_64_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15830220
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC15781719.969356079700724No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC3500972.211573812619155No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC2511201.5863329757893447No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT2024781.279059924625179No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1902551.2018468473590387No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1446980.9140618386857542No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA929250.5870101615770342No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG759080.47951323481290853No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC725120.458060595493935No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT621730.39274880576517573No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA443100.27990767026611124No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG207810.13127423371248156No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA173660.10970157079307805No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA171940.1086150413576059No Hit
GTCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT160810.1015841851850448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG568900.069.3099811
GCTGCCC3109650.069.16614512
CCTGTAC3105000.069.115712
GAGCTGC3115800.069.1147710
CTGTACT3102400.069.074563
GTACTGA3107450.069.064625
TCCTGTA3102500.069.058561
TGAGCTG3115000.069.056129
TGTACTG3104150.069.055734
TGTCTCG570950.069.0302610
ACTGAGC3121950.069.03027
TCTCGGT568100.069.0132912
CGTAAAT1134650.068.9284449
TGCCCCG3114550.068.9067414
TATTGGC1099650.068.875515
CAGCACG1134200.068.863874
CTGCCCC3116800.068.8637313
TTGGCGA1063050.068.8407617
AAATATT1133600.068.8165212
GTAAATA1136700.068.81644410
Produced by FastQC (version 0.11.5)