FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_62_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_62_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13643939
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC5144403.7704654059212666No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1206360.8841728184214251No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1205560.8835864774827855No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC685800.5026407696487063No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA601410.4407891298839727No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA528120.387072970642862No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG480210.3519584776800893No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT377660.27679689860823914No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG250600.18367129902882154No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC195440.1432430913096284No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA153920.11281199659423866No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG137610.10085797070772597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT272050.069.445318
GTTGTAT212150.069.421112
CGTAAAT1654250.069.403999
TTGGCGA1553900.069.38187417
TGGCGAA1552950.069.381518
GTCTCGG373450.069.37997411
TATTGGC1606500.069.3623615
AAATATT1653800.069.3445812
GTAAATA1657350.069.331210
GAGCTGC2911200.069.3264610
GCTGCCC2909000.069.3247512
CAGCACG1654500.069.313894
ATTGGCG1569600.069.3055416
GCACGTA1657400.069.2876366
TGAGCTG2911250.069.267579
ATATTGG1654150.069.26008614
CCTGTAC2902850.069.24282
TAGGTTG272600.069.228179
TCCTGTA2899200.069.2249151
TGTACTG2901000.069.217494
Produced by FastQC (version 0.11.5)