FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_5_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_5_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24315400
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3137991.2905360388889346No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2796431.1500653906577725No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC908290.3735451606800628No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA580950.23892265806854915No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA571710.23512259720177334No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC453110.18634692417151272No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG352740.14506855737516142No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT303350.12475632726584797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1839000.069.2175141
GTTGTAT836900.069.2127412
GTAGGTT1104100.069.206488
AGTAGGT1105500.069.150487
CGTAAAT3966450.069.0601049
CAGCACG3963050.069.058994
TATTGGC3856200.069.0476415
TTGGCGA3754600.069.0413917
TGGCGAA3753800.069.0130818
AAATATT3965300.069.0051112
GGTTGTA839950.069.0030811
ATTGGCG3790450.068.98276516
GTAAATA3974200.068.9790110
GCACGTA3971900.068.966796
AGGTTGT1049950.068.96545410
TAGGTTG1107200.068.9652949
ATATTGG3965600.068.9635614
GTATAGT1083350.068.93593615
GAGGTAG1903900.068.924872
TAGCAGC4414650.068.865121
Produced by FastQC (version 0.11.5)