FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_58_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_58_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17609299
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC5432653.0851029333989954No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1242090.7053602758406226No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC766850.4354801403508453No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT746580.4239691767400849No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA581520.33023461070199334No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT451160.2562055423103441No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA444550.2524518437673186No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG443340.25176470681768764No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG282890.16064807576951246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG491600.069.1655511
GTAGGTT287750.069.013318
TGTCAGT681700.068.9435041
GCTGCCC3963200.068.9308512
GGGCTAT153950.068.93001615
GAGCTGC3967300.068.9010810
GTTGTAT236350.068.8582912
TCTCGGT491800.068.8527612
TGTACTG3958000.068.835224
CCTGTAC3961950.068.8312
CTGTACT3957250.068.8272253
TGAGCTG3969150.068.814299
TCCTGTA3957750.068.802321
GTACTGA3964350.068.7973565
ACTGAGC3980750.068.735987
TGTCTCG494600.068.7177210
CGTAAAT1534050.068.684389
TGCCCCG3972900.068.6762214
GGCTATG142100.068.6694816
TGGCGAA1444950.068.6604218
Produced by FastQC (version 0.11.5)