FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_55_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_55_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26687210
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC18290606.853695084649164No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC4649701.742295279274229No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC2818531.0561351298993038No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA2562370.9601490751562265No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2470390.9256831268611444No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1853030.6943513390871507No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1612730.6043082060657521No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1198150.4489603821456046No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG859540.32207937809909687No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC786230.2946092903679328No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA575650.2157025781263759No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA269750.10107838174166577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT552900.069.157078
GCTGCCC4706400.069.1327512
GTCTCGG583900.069.0760211
TATTGGC2266900.069.07262415
TTGGCGA2208050.069.0578317
CGTAAAT2341600.069.0574659
GTTGTAT374750.069.0558312
GAGCTGC4711800.069.04893510
CAGCACG2339100.069.044024
GTAAATA2343200.069.0400510
TGGCGAA2207200.069.0224518
AAATATT2337500.069.006412
TGAGCTG4710350.068.993789
CCTGTAC4702550.068.982192
TCCTGTA4695050.068.976061
GTACTGA4701900.068.961755
ATATTGG2338400.068.9602514
CTGTACT4697350.068.9581763
TGTACTG4698150.068.95724
GCACGTA2344850.068.955326
Produced by FastQC (version 0.11.5)