FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_51_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_51_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22619118
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC5718492.5281666597256356No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3309421.463107447425669No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1322780.584806180329401No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA857630.3791615570509867No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC767170.3391688393862219No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA564670.24964280216408083No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG517930.22897886646154816No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA466720.2063387263818156No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT427410.18895962256353233No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT419520.1854714228910252No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG276700.12233014567588356No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG236330.10448241173683254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTAT537500.069.3217912
GTCTCGG679050.069.2722511
CGTAAAT3486200.069.268979
GTAGGTT872000.069.268348
TATTGGC3401950.069.25516515
CAGCACG3484600.069.254024
TGAGGTA1514200.069.233341
AGTAGGT873350.069.225397
GTAAATA3488750.069.2233610
AGGTTGT831800.069.2204110
AAATATT3481250.069.21580512
TTGGCGA3307900.069.2030317
TGGCGAA3305500.069.17583518
GCACGTA3492950.069.167636
GCTGCCC5062350.069.1617412
ATATTGG3483150.069.1579514
GAGCTGC5062050.069.1421810
ATTGGCG3334350.069.1336316
TCTCGGT677050.069.1150112
TAGCAGC3672500.069.111111
Produced by FastQC (version 0.11.5)