FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_47_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_47_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18972287
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT482290.2542076239938812No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC465660.24544220736277075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT2200000.069.5279549
AAATATT2197100.069.5002512
TATTGGC2126400.069.4869715
GAGCTGC4266250.069.4615110
CAGCACG2198700.069.4613654
TTGGCGA2066350.069.4604217
GTAAATA2203550.069.4604210
GCTGCCC4265100.069.4539812
TAGCAGC2396200.069.4511641
TGGCGAA2065050.069.42448418
CCTGTAC4261400.069.3979642
GCAGCAC2400950.069.394823
TGAGCTG4268100.069.392049
TGTACTG4256700.069.381914
TCCTGTA4256600.069.379181
CTGTACT4257050.069.3763963
GTTGTAT247150.069.3757612
GTCTCGG694200.069.3736911
GTACTGA4261250.069.3636865
ATTGGCG2086650.069.3583116
Produced by FastQC (version 0.11.5)