FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_412_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_412_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46065202
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3705840.8044770974845612No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC686300.148984476395002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGGC7665900.069.56280515
CGTAAAT7960200.069.554749
AAATATT7953100.069.52705412
TTGGCGA7432350.069.5250517
TGGCGAA7427650.069.5119518
GTAAATA7970500.069.5108910
GTAGGTT1486650.069.509138
CAGCACG7957850.069.507784
GTTGTAT1148150.069.4714612
ATTGGCG7476650.069.4693616
ATATTGG7952700.069.4605914
AGTAGGT1487850.069.460137
TGAGGTA2460450.069.459981
GAGCTGC10982500.069.4480610
AGGTTGT1397750.069.44536610
GCACGTA7971800.069.435336
TAGCAGC8621600.069.428961
GCTGCCC10982500.069.4232812
TGAGCTG10980050.069.3976449
GCAGCAC8636500.069.378543
Produced by FastQC (version 0.11.5)