FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_411_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_411_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18934367
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC4566102.411540876967263No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2960071.563331903305772No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1118670.5908145754225635No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA712330.3762100945862093No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC692650.3658162958392008No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA501290.26475139094958916No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT469060.24772943294064176No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG451060.23822290969642662No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC343310.18131580527619434No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG250610.1323572105684864No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT244750.12926230911231412No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA237660.12551779523445383No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA219230.115784171712738No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG189360.10000862452914322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG741100.069.31911511
GCTGCCC3131050.069.29494512
GAGCTGC3139550.069.14635510
CCTGTAC3132550.069.109092
GTAGGTT259000.069.107298
CTGTACT3131100.069.0616763
TCCTGTA3130100.069.049111
TGTACTG3130950.069.0430154
TGAGCTG3142150.069.040129
CAACGGA1497050.069.008671
TGCCCCG3137400.068.9940614
GTACTGA3136250.068.988835
TGTCTCG743950.068.9782910
TCTCGGT742900.068.9721312
CAGCACG604650.068.95224
CGTAAAT605050.068.946399
CTGCCCC3144400.068.87051413
GGCAGTT278850.068.84444411
ACTGAGC3156300.068.8167
TTGGCGA581200.068.8128517
Produced by FastQC (version 0.11.5)