FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_405_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_405_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26763863
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC7024762.624718262830743No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2109920.7883465850949842No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1182890.44197281984293524No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1110370.4148765819044882No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1038970.3881988186832372No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT817140.3053146700085858No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA783130.29260723685515805No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG763360.28522041082036625No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC493790.18449877732523143No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG278560.10408064037691421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7616950.069.1954812
GAGCTGC7621250.069.1890510
CCTGTAC7602100.069.18572
CTGTACT7592600.069.159463
TGTACTG7595550.069.144574
GTACTGA7604250.069.135885
GTCTCGG1122450.069.1354311
TCCTGTA7595000.069.130991
TGAGCTG7619900.069.12929
ACTGAGC7633500.069.101867
TGTCAGT1028050.069.0643541
TGCCCCG7618650.069.02051514
CTGCCCC7632550.068.9430913
CTGAGCT7647500.068.9044348
GCCCCGA7621150.068.8954615
TGTCTCG1126200.068.8368510
AGCTGCC7662850.068.7887811
TCTCGGT1124050.068.76312
GTAGGTT314550.068.675058
CATTGCA1126200.068.65941
Produced by FastQC (version 0.11.5)