FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_405_T2_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_405_T2_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18923792
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC668960.35350208879911593No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT350920.18543852098987348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC8294550.069.7487510
GCTGCCC8295900.069.7420412
CCTGTAC8259800.069.740822
TGAGCTG8289050.069.719039
TCCTGTA8250900.069.718461
CTGTACT8252300.069.711623
ACTGAGC8301300.069.704267
TGTACTG8254800.069.702174
GTACTGA8266150.069.696665
TGCCCCG8296150.069.60873414
CTGAGCT8309950.069.572748
CTGCCCC8305200.069.56238613
AGCTGCC8318850.069.5294411
GCCCCGA8299750.069.4950515
GTCTCGG532800.069.4077711
TATTGCA1530750.069.29991
GTAGGTT182200.069.288228
CCGAGAA4617650.069.2345618
CGAGAAC3330350.069.14493619
GGCAGTT230250.069.0565311
Produced by FastQC (version 0.11.5)