FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_402_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_402_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18842479
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1441150.7648409744811179No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1115790.5921673045250575No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC370540.19665140664346767No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA324230.1720739611810102No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA296330.15726699231030059No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC221540.11757476285365635No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG213150.11312205787784081No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA208280.11053747227209329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC3292950.069.5321610
GCTGCCC3291600.069.5309112
CCTGTAC3286850.069.463832
TGAGCTG3294850.069.434889
GTACTGA3289400.069.4317255
CTGTACT3285450.069.431493
TGTACTG3285150.069.4309844
GGCAGTT399900.069.4036111
TCCTGTA3286450.069.379591
ACTGAGC3304100.069.3782047
GTCTCGG762700.069.37466411
CGTAAAT865250.069.343649
AGTAGGT379150.069.288157
TATTGGC853850.069.2691315
GTAGGTT379100.069.260358
TGTCAGT818550.069.187931
AGGCAGT401150.069.1786310
TGCCCCG3304800.069.1568114
AAATATT867650.069.14358512
CAGCACG865850.069.12384
Produced by FastQC (version 0.11.5)