FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_402_T2_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_402_T2_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25843535
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT4178751.6169421095063041No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3008901.1642757076382932No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA1211610.4688251820039325No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC806630.312120613530618No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG525170.20321136408003007No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA521760.2018918851465173No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA512640.198362956151316No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC475190.18387190452080182No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT363710.140735390882091No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT304060.11765418314483679No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG292150.1130456804767614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6015300.069.5768912
GAGCTGC6021400.069.5453410
CCTGTAC6000000.069.506462
GTAGGTT486550.069.4953168
TGAGCTG6020000.069.486659
CTGTACT5994650.069.486593
TGTACTG5995950.069.483784
TCCTGTA5994700.069.470391
GTACTGA6004250.069.464685
ACTGAGC6034500.069.4090047
AGTAGGT487600.069.3887257
GTCTCGG802450.069.3575711
GGCAGTT528950.069.3436611
TGCCCCG6026600.069.33433514
CTGCCCC6034900.069.26797513
CTGAGCT6047900.069.188668
GCCCCGA6034850.069.1763415
TATTGGC960500.069.17155515
CGTAAAT975100.069.141019
TGTCAGT863500.069.080471
Produced by FastQC (version 0.11.5)