FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_39_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_39_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18316217
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1793220.9790340439840826No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT482680.26352603269550695No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC375500.20500958249184317No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA346380.18911110301870743No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC247500.13512615623630142No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA206230.11259421091156542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT2928950.069.588969
TATTGGC2822950.069.58015415
CAGCACG2927750.069.5560764
TAGCAGC3198250.069.546781
AAATATT2928150.069.5127312
TTGGCGA2752850.069.5117517
GTAAATA2933600.069.5037110
TGGCGAA2751700.069.4793718
ATATTGG2926900.069.4766514
GCACGTA2934000.069.475916
GCAGCAC3205450.069.451323
ATTGGCG2770950.069.4461616
GTAGGTT415700.069.434728
GAGCTGC2743950.069.40188610
GCTGCCC2743050.069.3803812
CACGTAA2939950.069.350027
AGCACGT2935650.069.3450555
GTCTCGG598150.069.34386411
GTTGTAT322150.069.32561512
CCTGTAC2742450.069.282
Produced by FastQC (version 0.11.5)