FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_399_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_399_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36008113
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1478530.4106102421973626No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1369080.3802143144796285No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC511190.1419652287805251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT5833650.069.621399
TATTGGC5616600.069.606915
TAGCAGC6407800.069.59171
CAGCACG5832850.069.584914
TTGGCGA5438850.069.5803217
TGGCGAA5436550.069.5691118
AAATATT5828350.069.5670912
GAGCTGC5797500.069.5616910
GTAAATA5844050.069.54240410
GCACGTA5841600.069.532816
GCTGCCC5796500.069.53091412
GTAGGTT1075750.069.530268
TGAGGTA2004350.069.525841
GTTGTAT858900.069.52209512
ATTGGCG5504300.069.5189816
ATATTGG5827000.069.50152614
GCAGCAC6423900.069.48923
TGAGCTG5801250.069.463639
CCTGTAC5786950.069.45822
TGTACTG5783250.069.4367144
Produced by FastQC (version 0.11.5)