FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_396_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_396_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34261424
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC585820.17098530405507956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG1639200.069.4693511
GAGCTGC6872850.069.4361610
GCTGCCC6866700.069.411712
TGTCAGT2118750.069.396161
CGTAAAT3738850.069.391439
TATTGGC3642150.069.36175515
TGTACTG6854300.069.355484
CCTGTAC6860950.069.3538062
GTACTGA6863300.069.344495
TGAGCTG6877250.069.3439949
CTGTACT6855050.069.33473
AAATATT3738300.069.32477612
CAGCACG3739550.069.316654
ACTGAGC6892100.069.3109747
TTGGCGA3502600.069.297717
TCCTGTA6858850.069.279491
GTAGGTT729450.069.274568
GTTGTAT590850.069.2702612
GTAAATA3748250.069.2593310
GCACGTA3746650.069.2356346
Produced by FastQC (version 0.11.5)