FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_393_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_393_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21220298
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3009201.4180762211727658No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT861260.4058661193165148No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC762520.35933519877996056No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC484030.22809764500008436No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA452750.2133570414515385No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT333660.15723624616393228No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA330480.155737680969419No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG308380.14532312411446813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATGG285050.069.213355
GCTGCCC5050750.069.130412
GAGCTGC5054950.069.1075910
CCTGTAC5043950.069.08842
TCCTGTA5039150.069.055561
TGAGCTG5056100.069.055349
CTGTACT5042050.069.0400853
TGTACTG5042700.069.039284
GTACTGA5050400.069.019265
ACTGAGC5067650.069.0098347
GTCTCGG652100.068.9789111
TGTCAGT748450.068.9145661
CGTAAAT1337800.068.8948069
TATTGGC1297900.068.8930715
TGCCCCG5059350.068.8856114
GTAGGTT342200.068.853338
AGTAGGT342350.068.8231667
CTGAGCT5077100.068.8041848
TTGGCGA1260050.068.8023317
CTGCCCC5068750.068.7875513
Produced by FastQC (version 0.11.5)