FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_392_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_392_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18678912
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC6244873.343272884416394No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1386940.7425164806172865No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC959180.5135095662959385No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA851230.45571712099719724No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA784410.4199441594885184No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT716410.38353946953655543No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG620700.3322998684291676No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT432040.23129826833597159No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC317670.1700687920152951No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG314200.16821108210156993No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG231480.12392584750118209No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG198960.10651583989474334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4287600.069.256412
GAGCTGC4289850.069.2478210
GTCTCGG600500.069.2473711
TGAGGTA971150.069.244491
CCTGTAC4276600.069.2186052
GTAGGTT546200.069.210518
TGAGCTG4287800.069.1987469
TGTACTG4274450.069.187984
CTGTACT4272850.069.1867753
AGTAGGT546700.069.179237
GTACTGA4278250.069.17075
ACTGAGC4297100.069.1621867
CGTAAAT2510700.069.142759
TCCTGTA4276700.069.127771
GTTGTAT429600.069.127512
TATTGGC2435100.069.1224815
CAGCACG2509700.069.116654
TTGGCGA2354750.069.0853217
AAATATT2508700.069.0812412
TGGCGAA2352950.069.075718
Produced by FastQC (version 0.11.5)