FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_386_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_386_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24162678
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT4932312.041292773921831No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC503180.20824678456584986No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG289110.11965147240715619No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA263430.10902351138396167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7461350.069.6982812
CCTGTAC7432950.069.6607362
GAGCTGC7465500.069.6604810
TCCTGTA7426900.069.635471
CTGTACT7428750.069.6196443
TGAGCTG7463450.069.619589
TGTACTG7430550.069.615334
GTACTGA7446450.069.5573965
TGTCAGT1362050.069.555571
TGCCCCG7474400.069.5246114
CTGCCCC7478050.069.4831913
GTAGGTT341350.069.476058
ACTGAGC7485750.069.47537
AGCTGCC7490150.069.4200111
CTGAGCT7486550.069.408058
GCCCCGA7485250.069.3831615
GGCAGTT417900.069.33732611
GTCTCGG644350.069.3199911
AGTAGGT343050.069.182777
CAACGGA2082300.069.170871
Produced by FastQC (version 0.11.5)