FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_385_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_385_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178233
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT5950.3338326796945571No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2260.12680031195120994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGATG500.070.0143160
GGATGGG455.456968E-1270.0143162
GTTAGTA600.070.0143152
CTTGGAT500.070.0143159
GATGGGA455.456968E-1270.0143163
TGGATGG500.070.0143161
GGTTAGT600.070.0143151
AGTACTT600.070.0143155
GTAGGTT3350.069.9946758
GTTGTAT2500.069.99467512
TCATGGT1150.069.99467511
AGTAGGT3350.069.9946757
GTTTGTG950.069.9946711
CAGGTGT207.913259E-569.994676
GTGCAGG700.069.9946714
TAATATC252.8324284E-669.9946741
TATCAAA150.002222483769.9946741
TTAGGGA150.002222483769.9946740
GGCAGTT600.069.9946711
CCTTATG150.002222483769.9946747
Produced by FastQC (version 0.11.5)