FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_384_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_384_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17757897
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1054990.594096249122292No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC332370.18716743317071838No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG194240.1093823215665684No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA184050.10364402947038154No Hit
AAATGTGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTC181960.10246708830443155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT792150.069.738498
TAGCAGC3914150.069.725821
CGTAAAT3713400.069.692849
CAGCACG3711050.069.6721344
TATTGGC3618750.069.66122415
AAATATT3709700.069.6527112
GTAAATA3718350.069.6497610
GCAGCAC3922400.069.646883
AGGTTGT758550.069.6344610
GCACGTA3718250.069.626446
GAGCTGC3045700.069.6174510
AGTAGGT794550.069.615937
TGAGGTA1398100.069.6158751
GCTGCCC3047600.069.6004612
TTGGCGA3500450.069.58182517
GTTGTAT600000.069.5789712
TGGCGAA3496400.069.5783518
ATATTGG3710900.069.5752914
ATTGGCG3542050.069.5432516
CACGTAA3724400.069.5170757
Produced by FastQC (version 0.11.5)