FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_381_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_381_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22534858
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3102371.3766982689662388No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1644610.7298071281389925No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC704940.31282202887633015No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC458380.2034093136952538No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA421120.18687492949811355No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT357260.15853661025953658No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA325150.144287574388088No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG279270.12392800522639193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6834900.069.67940512
GAGCTGC6835900.069.6528310
CCTGTAC6821800.069.614522
TCCTGTA6813800.069.605821
TGAGCTG6835350.069.6026159
TGTACTG6817600.069.588864
CTGTACT6817200.069.580373
GTACTGA6823850.069.56825
ACTGAGC6854750.069.509797
TGCCCCG6843300.069.4960314
CTGCCCC6852450.069.4238213
GCCCCGA6846450.069.4114115
TGTCAGT1228850.069.402521
AGCTGCC6867650.069.32571411
CTGAGCT6867300.069.309388
CGTTACC1442100.069.286025
GTTACCA1442050.069.259296
TACCATT1442450.069.161838
CATTACT1438250.069.1618211
GTCTCGG515150.069.1564711
Produced by FastQC (version 0.11.5)