FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_37_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_37_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15356919
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC5898193.8407378459181816No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1245970.811341129037667No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC893710.581959180744523No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA760390.49514489201903067No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT600860.39126337776477166No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG561330.36552253743084795No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA504030.3282103656338879No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT363380.23662298407642834No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG281410.18324639206601273No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC251020.16345726639568783No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG214360.13958529051302543No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG211980.1380355004802721No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA169760.11054300670596752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG637950.069.1154211
TGTCAGT711350.068.942071
GCTGCCC2915600.068.9087212
GAGCTGC2917400.068.897410
TGAGGTA641750.068.85791
CCTGTAC2918600.068.839552
CTGTACT2870800.068.83793
TGTACTG2871300.068.8191154
TGAGCTG2874500.068.816439
GTAGGTT368000.068.809868
TCTCGGT637750.068.80781612
GTACTGA2874650.068.801015
TCCTGTA2915350.068.7951
GTTGTAT300750.068.78841412
ACTGAGC2883350.068.784857
TATTGGC1528050.068.7366615
AGTAGGT368850.068.717717
CGTAAAT1585800.068.711999
CAGCACG1586150.068.707224
TTGGCGA1482950.068.6968117
Produced by FastQC (version 0.11.5)