FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_367_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_367_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25288097
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2907961.1499323179597105No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA493100.19499292493223194No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC412800.16323885502337326No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT394270.15591129692360797No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG360790.14267186653072392No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA294260.1163630462189385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC5683400.069.7500110
GCTGCCC5681900.069.7426912
CGTAAAT3403650.069.681199
TAGCAGC3544450.069.671011
CCTGTAC5675300.069.6694262
TGAGCTG5688250.069.646379
TATTGGC3334700.069.645115
CAGCACG3403000.069.638154
TGTACTG5672550.069.634914
CTGTACT5672000.069.634893
GTACTGA5677950.069.633895
AAATATT3403450.069.6163912
TTGGCGA3233150.069.6162717
GCAGCAC3548750.069.613823
GTAAATA3411750.069.5935810
ACTGAGC5702700.069.578057
GCACGTA3408700.069.569826
TGGCGAA3232700.069.5554518
ATTGGCG3278300.069.5389316
ATATTGG3405750.069.5240214
Produced by FastQC (version 0.11.5)