FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_365_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_365_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25091903
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1384540.551787562704989No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC369250.147159025762215No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT356940.14225306067857826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT3422850.069.4354259
TATTGGC3310850.069.4184815
CAGCACG3420400.069.386464
TGTCAGT1558100.069.382111
TTGGCGA3209400.069.3779317
AAATATT3419300.069.376512
GTAAATA3428450.069.3628510
TGGCGAA3207600.069.3361218
ATATTGG3419050.069.3303814
GCACGTA3427450.069.328496
TAGCAGC3846150.069.319411
ATTGGCG3246900.069.308316
GAGCTGC3524300.069.29970610
GTAGGTT574000.069.285438
TGAGGTA1016450.069.268341
GCTGCCC3524950.069.2670612
GCAGCAC3855200.069.24973
AGCACGT3428150.069.2316365
CCTGTAC3517750.069.220572
TGAGCTG3527050.069.18919
Produced by FastQC (version 0.11.5)