FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_352_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_352_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15162315
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3400532.2427511893797223No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC979000.6456797659196502No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT775930.511749030408615No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA618850.4081500747082487No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC583890.38509290962494847No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT442030.29153199890649945No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG376450.24828002847850078No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC327600.21606199317188698No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA320010.21105616127880208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAC4639950.069.399162
GCTGCCC4654050.069.384512
GAGCTGC4654000.069.3702110
TCCTGTA4636950.069.333071
CTGTACT4637600.069.331253
TGTACTG4637200.069.320994
GTACTGA4643600.069.297815
TGAGCTG4654950.069.275599
ACTGAGC4664300.069.274047
TGCCCCG4657800.069.217214
CTGCCCC4663300.069.1703113
CTGAGCT4667150.069.13958
GCCCCGA4663550.069.0680615
AGCTGCC4678000.069.0038311
GTAGGTT160450.068.886428
GTCTCGG327500.068.780611
TGTCAGT474100.068.751
CCGAGAA2287150.068.7320618
AGTAGGT161000.068.607617
CCGAAAC1882800.068.5305918
Produced by FastQC (version 0.11.5)