FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_346_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_346_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511110
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1424750.7696729153465135No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC357270.1930030127852949No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT313610.16941717703584494No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC211790.11441237181346768No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA195050.10536915398374275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT1740200.069.555761
GAGCTGC3631000.069.44533510
GCTGCCC3629300.069.4257912
TGAGCTG3633150.069.371499
CCTGTAC3628450.069.3671952
CTGTACT3625250.069.357863
TGTACTG3626100.069.348794
GTACTGA3630900.069.33235
GTCTCGG656650.069.3110511
ACTGAGC3643750.069.307037
TCCTGTA3627500.069.299471
GGCAGTT384750.069.2163111
TGCCCCG3636550.069.1507214
CTGCCCC3643200.069.0417813
GTAGGTT204350.069.03958
CGTTACC2090600.069.0226065
CTGAGCT3655100.069.014268
CAACGGA1334950.068.9904561
CCGTTAC2090850.068.985894
AACCGTT2088700.068.969222
Produced by FastQC (version 0.11.5)