FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_335_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_335_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23867942
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC4630621.9401002398950022No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1549820.6493312242840208No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT862470.3613508026791753No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC729160.3054976419835443No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA709150.2971140117568578No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG486350.20376704451519112No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA447870.18764500098081352No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT433920.18180034122757632No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC239870.10049881971390746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4611300.069.4117812
GAGCTGC4617000.069.383710
CCTGTAC4610950.069.304542
TGAGCTG4617600.069.300559
TCCTGTA4607050.069.272781
CTGTACT4606800.069.270673
TGTACTG4608400.069.262964
TGTCAGT1475900.069.262331
GTACTGA4614050.069.2289665
ACTGAGC4635800.069.144757
GTAGGTT238000.069.1162958
TGCCCCG4624200.069.1114314
CTGCCCC4627350.069.0666513
GTCTCGG635450.069.0511411
CGTTACC3126900.068.949745
GGCAGTT366300.068.9474511
GTTACCA3126150.068.923746
CTGAGCT4647300.068.8990868
CCGTTAC3127950.068.88524
GCCCCGA4636650.068.86320515
Produced by FastQC (version 0.11.5)