FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_332_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_332_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21668327
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC607980.2805846524284039No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT285920.13195296526584632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC6236300.069.4910
GCTGCCC6233750.069.4690212
CCTGTAC6219250.069.462252
TGAGCTG6235550.069.4237069
CTGTACT6213650.069.413623
TGTACTG6215250.069.407594
ACTGAGC6245550.069.407267
GTACTGA6224150.069.389315
TCCTGTA6216450.069.388791
GTCTCGG696300.069.2796811
CTGAGCT6251900.069.262868
TGCCCCG6242050.069.2465614
TGTCAGT888950.069.1971
CTGCCCC6249300.069.17966513
GCCCCGA6247500.069.09820615
GTAGGTT262600.069.0788358
GGCAGTT366800.068.9870411
AGCTGCC6280950.068.97706611
CATTGCA699500.068.924021
TGTCTCG700200.068.91379510
Produced by FastQC (version 0.11.5)