FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_331_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_331_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69895
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC11751.681093068173689No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3700.5293654767866085No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC3040.43493812146791616No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1980.28328206595607697No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1800.2575291508691609No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1750.25037556334501754No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1220.1745475355890979No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1160.1659632305601259No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG810.1158881178911224No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG760.10873453036697904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT8900.070.0481
AACCGTT8900.070.0482
TGGCAAT353.6270649E-970.0483
CACATTG252.8016893E-670.0483
CAGCATT353.6270649E-970.0483
CCTGCCT207.849951E-570.0482
ACCGTTA8950.070.0483
TGTCAGT4650.070.0481
CTGACCT207.849951E-570.0481
TATTGCA4300.070.0481
TCTACGG252.8016893E-670.0482
TCTAATT252.8016893E-670.0483
GCAGCAT353.6270649E-970.0482
GCTGCCA252.8016893E-670.0483
GGGGGAT353.6270649E-970.0481
CCCTGGT207.849951E-570.0482
TGTAGTG700.070.0481
CAAAGTG750.070.0481
TGAGGTA1750.070.0481
CTACGGC252.8016893E-670.0483
Produced by FastQC (version 0.11.5)