FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_326_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_326_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23078655
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2162900.9371863308325377No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT829410.35938402822868143No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC677570.29359163261463894No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC387720.167999391645657No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA386850.1676224199373837No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT308100.133499980826439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG872950.069.2330511
TGTCAGT1099350.069.174041
GTAGGTT943200.069.138098
TGAGGTA1719200.069.1178741
GTTGTAT775900.069.110212
TATTGGC4177050.069.10715
TTGGCGA4052150.069.0935317
CGTAAAT4337500.069.069429
AGTAGGT944600.069.0578467
TGGCGAA4050150.069.0541918
AAATATT4332600.069.0464112
GTATAGT1021500.069.0460315
ATTGGCG4093750.069.0367516
GTAAATA4344850.069.0305710
ATATTGG4331400.069.02241514
CAGCACG4336200.069.011124
AGGTTGT888950.068.9987910
GCACGTA4342650.068.994866
TCTCGGT872100.068.98749512
GGTTGTA778450.068.93326611
Produced by FastQC (version 0.11.5)