FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_324_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_324_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24294478
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3555081.4633284156177384No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3425341.4099253336498936No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1051050.4326291760621488No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA522040.21488010567668916No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC511350.210479928813453No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG343050.1412049273089959No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT315560.1298895987804307No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC285390.11747113891477726No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC256400.1055383861303791No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5399400.069.6300212
GAGCTGC5400450.069.6138910
CCTGTAC5385000.069.5339662
TGAGCTG5399250.069.531499
TCCTGTA5379400.069.510031
TGTACTG5380100.069.507774
CTGTACT5379500.069.501623
GTACTGA5388850.069.4527055
GTTACCA2572400.069.439016
CGTTACC2573350.069.433785
TGCCCCG5412950.069.41627514
TGTCAGT1293850.069.382441
CCGTTAC2573350.069.378014
CTGCCCC5416400.069.3339413
CATTACT2572200.069.32123611
TACCATT2576550.069.3060768
ACTGAGC5422250.069.303677
CTGAGCT5422250.069.24888
TTACCAT2577000.069.226077
CAACGGA2282600.069.2115551
Produced by FastQC (version 0.11.5)