FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_321_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_321_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33979846
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2145720.63146842984515No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC448020.1318487435169659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC8438650.069.71464510
GCTGCCC8433850.069.7032812
TGAGCTG8440050.069.659959
CCTGTAC8422950.069.6547242
CGTAAAT3688850.069.6429
TATTGGC3612000.069.6305415
TGTACTG8418300.069.6241464
CTGTACT8416950.069.6229253
TAGCAGC3894900.069.622361
GTACTGA8427650.069.613765
ACTGAGC8461650.069.591747
GCAGCAC3900450.069.587723
CAGCACG3688550.069.5778664
TGGCGAA3470200.069.56174518
TTGGCGA3473700.069.5561417
GTAGGTT860850.069.5474558
GTAAATA3697000.069.5291810
AAATATT3689750.069.5282612
ATATTGG3689150.069.49402614
GTTGTAT617650.069.4887612
Produced by FastQC (version 0.11.5)