FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_319_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_319_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18935196
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1293260.682992666144042No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT430460.22733326869180545No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC339950.1795333937921741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC5281850.069.6786710
GCTGCCC5279550.069.66196412
CCTGTAC5270350.069.6267552
TGAGCTG5282400.069.613789
TGTACTG5266000.069.598954
GTACTGA5273100.069.588875
CTGTACT5266350.069.58663
ACTGAGC5297650.069.546187
TGCCCCG5287650.069.45975514
CTGCCCC5292050.069.4028513
CTGAGCT5307000.069.343198
TGTCAGT772000.069.309241
CGTTACC2311000.069.303735
TCCTGTA5288850.069.297151
CCGTTAC2309650.069.294234
GCCCCGA5295800.069.2834715
GTTACCA2310700.069.265766
AACCGTT2308800.069.232742
GTCTCGG515500.069.2247511
TACCATT2305300.069.1333548
Produced by FastQC (version 0.11.5)