FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_318_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_318_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27805510
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2808991.0102278289446949No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2672790.9612447317096503No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC652860.23479518987423714No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA462250.16624402861159532No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC364730.13117184327854442No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA335680.1207242737140948No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA287530.10340756202637535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT1055250.069.272528
GTCTCGG873650.069.2577511
GTTGTAT598200.069.2441312
GAGCTGC9284100.069.2437910
TGAGGTA1731450.069.234911
GCTGCCC9288250.069.2226512
AGGTTGT1014300.069.2121410
AGTAGGT1056750.069.207327
TGAGCTG9276800.069.19839
CCTGTAC9264200.069.16942
ACTGAGC9297900.069.1647347
GTACTGA9260200.069.1615755
TGTACTG9253250.069.154524
GGGCTAT394250.069.14664515
CTGTACT9252600.069.1463853
AGTAGTT491400.069.1441967
TATTGGC2476450.069.14384515
TCCTGTA9254600.069.133341
CGTAAAT2527200.069.1305549
TTGGCGA2398450.069.07896417
Produced by FastQC (version 0.11.5)