FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_317_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_317_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22150197
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1165820.5263248900224229No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT964210.43530538351419623No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC278390.12568285510056637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG740100.069.4787111
TATTGCA1977750.069.327661
CGTTACC2450400.069.3255165
GTTACCA2449800.069.321066
CCGTTAC2450400.069.271234
TGTCAGT835650.069.242971
TACCATT2451400.069.227928
CATTACT2443450.069.20248411
TTACCAT2452750.069.154147
AACCGTT2453000.069.141722
GTAGGTT243450.069.121938
CGTAAAT972000.069.11679
TCTCGGT741250.069.1159412
TATTGGC952050.069.0798415
AAATATT971900.069.07339512
TGTCTCG744300.069.06312610
CCATTAC2451100.069.0607510
GGCAGTT290500.069.04710411
TAGCAGC1056850.069.044121
ACCGTTA2458500.069.02593
Produced by FastQC (version 0.11.5)