FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_314_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_314_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21949314
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1154470.5259708800010788No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT899860.4099718105085198No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC276460.12595382252037582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5326500.069.4443112
GAGCTGC5327950.069.4293610
TGTCAGT1080100.069.4236151
TGAGCTG5326450.069.384019
CCTGTAC5315550.069.3838352
CTGTACT5311350.069.3670043
TGTACTG5313450.069.353814
TCCTGTA5311650.069.3372341
GTACTGA5317100.069.333855
ACTGAGC5341850.069.294717
GTAGGTT402550.069.2687158
TGCCCCG5335950.069.2017914
GTCTCGG590300.069.1984611
TATTGGC1075000.069.1880415
CGTAAAT1106400.069.186069
CTGAGCT5346550.069.155248
CTGCCCC5341700.069.1484213
CAGCACG1107100.069.095844
GCCCCGA5341000.069.085915
CAACGGA1683300.069.035111
Produced by FastQC (version 0.11.5)