FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_313_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_313_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18023139
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3077531.7075438412809223No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1013900.5625546138217099No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC788260.4373599959474318No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC549350.304802620675566No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA526570.29216331295009157No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT363700.2017961466090896No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG338310.18770870046555152No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA303590.16844457560916554No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC200370.11117375280743272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT415850.069.283078
CGTAAAT2155700.069.282469
CAGCACG2154650.069.258334
TATTGGC2085250.069.2381415
TGGCGAA2022350.069.2329518
AAATATT2154250.069.22674612
TTGGCGA2025000.069.2166617
GTAAATA2159500.069.20592510
GCACGTA2158600.069.186726
GTTGTAT304800.069.1831912
GCTGCCC3035250.069.1278812
GTCTCGG554550.069.127511
ATTGGCG2048950.069.1261716
TAGCAGC2406800.069.122241
GAGCTGC3036000.069.11771410
ATATTGG2157950.069.11291514
AGTAGGT417000.069.108797
TGAGGTA715200.069.088221
TGAGCTG2953550.069.062329
CCTGTAC3032100.069.024152
Produced by FastQC (version 0.11.5)