FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_311_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_311_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20355783
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1383860.6798362902571716No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC347450.17068859498060085No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC206980.10168117826762056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC4821050.069.5502610
GCTGCCC4815900.069.544712
TGAGCTG4819700.069.4929359
CCTGTAC4811200.069.48722
CTGTACT4805900.069.465483
TGTACTG4806850.069.4651644
ACTGAGC4831350.069.452147
GTACTGA4811950.069.451185
TCCTGTA4809200.069.428021
TGTCAGT1173100.069.4178161
GTCTCGG700950.069.3847511
TGCCCCG4820850.069.2896114
GGCAGTT278400.069.2697611
CTGCCCC4828550.069.2523213
GTAGGTT229250.069.235748
CTGAGCT4843700.069.203538
TATTGCA1405050.069.1731
CGTTACC2134700.069.156865
GTTACCA2134400.069.132156
GCCCCGA4827800.069.1318715
Produced by FastQC (version 0.11.5)