FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_304_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_304_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31753987
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC4179881.3163323396208482No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1102920.34733276170957683No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1026270.3231940606387475No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA699540.22029989493917726No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC618160.19467161714212455No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA608080.19149721261774152No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG415470.13084026267315663No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT398130.12537953108061675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5636500.069.46290612
GAGCTGC5642900.069.43870510
CCTGTAC5634200.069.355292
TGAGCTG5644450.069.3546459
TGTACTG5627200.069.348174
TGTCAGT1684300.069.315521
CTGTACT5630150.069.313023
GTACTGA5637600.069.308395
TCCTGTA5630050.069.301321
GTCTCGG967300.069.25703411
ACTGAGC5665650.069.235977
CGTTACC5165600.069.125945
TGCCCCG5654550.069.1235714
GTTACCA5165700.069.117156
CCGTTAC5164400.069.105414
AACCGTT5163650.069.0499342
TACCATT5160400.069.030388
CTGCCCC5665100.069.01214613
CATTACT5131250.069.0111211
TTACCAT5157900.069.009557
Produced by FastQC (version 0.11.5)