FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_302_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_302_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23599276
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT665880.2821611985045643No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC365890.15504289199380522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC7282250.069.532510
GCTGCCC7279450.069.5063612
CCTGTAC7271050.069.478542
TGAGCTG7283450.069.47789
TGTACTG7264600.069.472514
CTGTACT7264950.069.456523
GTACTGA7272300.069.456235
ACTGAGC7297850.069.4538967
GTCTCGG947950.069.4301211
TCCTGTA7267100.069.4213941
CTTGTCT947450.069.3928768
TGTCAGT985450.069.318411
TGCCCCG7292200.069.30276514
CTGAGCT7311450.069.253858
GTAGGTT248850.069.225158
CTGCCCC7301000.069.20724513
GCCCCGA7295750.069.2066715
CATTGCA950450.069.185831
TGTCTCG951300.069.1635410
TCTCGGT948450.069.1573412
Produced by FastQC (version 0.11.5)