FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_298_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_298_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26815772
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1968550.734101557844391No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1026780.38290152526654836No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC530930.19799168936848063No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC326850.12188722368313691No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA324950.12117868543930044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG1019700.069.2852211
TGTCAGT1442700.069.2718051
GAGCTGC5588300.069.22002410
GCTGCCC5584350.069.2125812
TATTGGC3421700.069.1704915
TGAGCTG5586950.069.167219
GTAGGTT535200.069.1424568
CGTAAAT3534250.069.1395659
TGGCGAA3315700.069.1325218
CAGCACG3533650.069.113194
TTGGCGA3319050.069.1091417
CTGTACT5576350.069.103423
CCTGTAC5586950.069.099412
GTTGTAT447150.069.0990112
AAATATT3532300.069.09846512
TGTACTG5577300.069.095384
ATTGGCG3347550.069.09057616
TCTCGGT1019550.069.0894412
TCCTGTA5579750.069.088811
GTACTGA5584800.069.0834355
Produced by FastQC (version 0.11.5)